Laser Microscopy for Biologists – 3 Day Workshop
An imaging workshop will be held at the Australian National University in Canberra in November this year. This will be hands-on opportunities for working with optical setups and demonstration of their application in-vivo and intra-vital imaging techniques.
Topics that will be covered are microscope optics, laser scanning, lasers coupling, optoelectronics devices, digital imaging and computing resources that are now used in advanced in vivo microscopy systems.
This course is designed primarily for research scientists, postdoctoral trainees, and advanced graduate students in animal, medical, and material sciences. Some prior theoretical or practical understanding of the basic principles of optics and microscopy is necessary. This 3 day course is limited to 16 students with short lectures, laboratory exercises, practical demonstrations, and discussions. Through a series of hands-on opportunities for researchers will have the opportunity to learn, understand and innovate thereafter. At the end of the course, the attendees will gain an in-depth and practical understanding of the physics and engineering of a laser scanning microscope. Students will have direct hands-on experience with state-of-the-art microscopes, digital cameras, recorders, and image processing equipment provided by major optical, electronics, and software companies. Instructions will be provided by experienced staff from ANU and the Garvan institute. Students are encouraged to discuss individual research problems with the faculty.
Monday 19 November 2018 Lectures: 9am – 1:00pm and 2 – 5pm
- Making an Airy Disk: Diffraction limits and optical aberrations
- Depth sectioning: confocal, pinhole and 2 photon
- Matching planes: conjugate scanning planes
- Analogue to Digital: Camera, PMT
- Sampling: Rates, Dynamic Range, Pixel Resolution, Signal to noise and DAQ hardware
- Dimensions in Imaging: 1D, 2D, 3D, 4D
- Talks from Industry – TBC 2- 5pm
Tuesday 20 November 2018 Hands-on sessions: 9am – 5pm
Full day hands-on
- In-vivo: 2 photon mouse intra-vital, 2 different organs
- 2 photon brain slice
- Holographic and microfluidic in-vivo imaging
Wednesday 21 November 2018 Follow-up session: 9am – 2:30pm
- Discussion of results from hands-on vs theory
- Improving images: deconvolution
- Follow up question and answer session
Lecturer and demonstrators:
Dr Steve Lee, Mr Yongxiao Li, Mr Thomas McMenamin, Mr Avinash Upadhya, Ms Yujie Zheng
Michael Castanares (Dr Vince Daria group, JCSMR)
Garvan Institute (Tri Phan lab)
Cost: $550 for LMA members, $600 for non-members
(Morning tea and lunch provided each day.)
Prerequisite for attendees: Basic microscopy knowledge.
For further course information: Steve.Lee@anu.edu.au
Seminar and lab location: JCSMR Building 131, ANU