Super Resolution Category

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Live Cell Category Winners
Life Science Category Winners
In Vivo Category Winners
Materials Science Category Winners
Volume Imaging Category Winners

1ST PRIZE: Edward Buckley, Centre for Cancer Biology, University of South Australia

Armed and Ready. Bone marrow derived macrophage, treated with 100 ng/ml LPS for 24 hours. Probed with CD206 (green), F4/80 (red), DAPI (blue). Imaged on the LSM800 at 63xOil using airyscan.

2ND PRIZE: Esther Miriiklis, ARMI at Monash University

Prolonged Nuclear Stress Dramatically Alters DNA Organisation. The effect of prolonged transcriptional inhibition by actinomycin D results in extensive DNA compaction shortly before cell death in human induced pluripotent stem cells. DNA was labelled with EdU for 24 hrs and treated with 4 µM actinomycin D during the last 4 hours. Alexa Fluor 647 was covalently attached to EdU via an alkyne-azide click reaction and imaged via direct stochastic optical reconstruction microscopy (dSTORM) on a bespoke microscope setup. The data was rendered through the rapidSTORM algorithm with a final pixel size of 20 nm and pseudo-coloured based upon localisation density (from low, blue, to high, white).

DISTINCTION: Sonja Frolich, Research Centre for Infectious Diseases, The University of Adelaide

Living in a Bubble. Malaria parasites were induced to invade human red blood cells (RBCs) in vitro and allowed to develop over 48 hr period with images captured at 10, 30 and 44 hours post invasion. These are 3D rendered models of Expanded parasites labelled with NHS Ester to reveal rhoptry content (club-shaped organelle housing proteins critical for RBC invasion) and Dapi to highlight nuclear division as the parasite matures into invasive daughter merozoites. Micrographs were captured using 63X oil objective on a LSM800 equipped with Airyscan detector (Z-stack – 50 slices, 250 nm step size, 15 µm range). Z-stacks reconstructed and rendered using Imaris with rhoptry volume statistically coded to reveal differences in volume during rhoptry biogenesis.