Volume Imaging Category

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Live Cell Category Winners
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1ST PRIZE: Kate Brody, University of Melbourne

The Cochlear Implant in Motion. Here is a cochlea which has been implanted with a Cochlear Implant for 6 weeks. It has been prepared for Lightsheet microscopy and labelled with Myosin VIIa to reveal the hair cells and IBA1 to detect macrophages both in the green. Fibronectin which labels the extracellular tissue response to the implant and HuC/D which shows the sprial ganglion neurons both in red. Smooth muscle actin labels fibrosis and arteriole in the blue. The image is imaged at 1.25X magnification on the Ultramicroscope II by La vision. Post-imaging the clearing media was removed and the electrode contacts were imaged using a Bruker Skyscan and reconstructed using Nrecon the images were over-layed using Imaris 9.6.

2ND PRIZE: Dillon Jevon, University of Sydney

Capillary Segmentation. Video showing manual segmentation of the capillary network in a pancreatic islet.

DISTINCTION: Veronika Valova, University of Sydney

Amyloid Plaques in Cleared Mouse Brian Hemisphere. Amyloid plaques (a feature of Alzheimer’s Disease) in optically-cleared mouse brain hemispheres were stained with the fluorescent dye Congo Red and imaged in three-dimensions using the La Vision Biotech UltraMicroscope II (~10 hours per dataset). The plaques were then segmented and quantified using a deep-learning approach, and registered to two brain regions of interest shown in blue (hippocampus) and red (cortex).

DISTINCTION: Jiahong Li, Bio21 Institute, University of Melbourne

Expansion Microscopy of Plasmodium Falciparum Mitosis Schizont. I applied the new development expansion microscopy technique to acquire images via Zeiss Elyra/LSM880 microscope. Expansion microscopy can enlarge the cell size 4-5 times, which can explore the ultrastructure of the malaria parasite, Plasmodium falciparum, cells using fluorescence microscopy. We used anti-tubulin to label the microtubules (red), DAPI to stain the chromosomes (blue), and NHS ester 488 to mark the general proteins (nuclear membrane, parasite membrane, etc.) (green). The movie indicated that the parasite mitosis is unsynchronized in the single cell, and the different structures of spindle microtubules indicated the various stage of mitosis.