We are pleased to announce the winners of the 2020 Light Microscopy Australia Image Competition.

We had 116 eligible entries across the four categories and with so many beautiful images this year, it was very competitive.

Each category will award a $400 first prize and a $200 second prize. We would like to thank the sponsors of each category, Optiscan, Zeiss Australia and Nikon for their support of the competition.

Please follow the links below to see this years winners.

Live Cell Category Winners
Life Science Category Winners
Materials Science Category Winners

 

IN VIVO CATEGORY

Proudly sponsored by OptiScan

 

 

 

1ST PRIZE: Arnolda Jakovija – Garvin Institute of Medical Research

2 Photon of Mouse Ear Skin – 2-photon in vivo imaging of Mouse ear pinnae. The image shows the accumulation of myeloid cells expressing GFP (in green) under LysM promoter, 24 hours after skin injury. In blue collagen visualized by Second Harmonic generation Imaging and the other skin components in purple. Multiple z-stacks were captured to cover the whole ear skin and stitched together. This transgenic mouse model allows us to study in real time myeloid cell migration after tissue damage. This approach will suggest specific elements to target during wound healing to improve abnormal scars formation, fibrosis and non-healing wounds (tumours).

---

2ND PRIZE: Hannah Hanh Truong – Peter MacCallum Cancer Centre

Human Lungs Grow in Fly Brain – I generated brain tumour models in Drosophila fruit flies to study about the behaviours of cancer stem cells during tumour progression. I knocked down the transcription factors prospero (pros, human homolog Prox1) in neural lineages to allow immature progenitor cells revert back to neural stem cell fate and form tumours. I used grainy-head gal4 driver to induce the expression of a GFP reporter gene and a RNAi against pros in neural lineages to generate the tumours and label them with GFP. I stained the tumours and neural circuits with GFP antibody. I took the image by FV3000 with 60X lens immersion oil and this is a maximum intensity projection image. The lung is actually a cluster of 3 tumours merged and fused together on each lateral size of the brain.

---

DISTINCTION: Daniel Cultrone – Garvan Institute of Medical Research

A Fish Made of Lights – The video navigates through the 3D rendering of a double-positive NF-KB (in green) Macrophages (in red) and Collagen (in magenta) zebrafish.
It switches between the overlapping of the green and red signals and the individual macrophages to better show the colocalization of NF-KB in macrophages in a more cell-sparse area of this zebrafish.

---

DISTINCTION: Harriet Manley – ARMI

Expect the Unexpected: The Neutrophil Edition – The unexpected, expulsive behaviour of an unusually round, single neutrophil as revealed by in vivo lattice light-sheet microscopy (3i LLSM). Live imaging was performed in the tail of a transgenic Tg(mpx:mCherryCaaX; eGFP-lmnB2) zebrafish larva, with genetically-labelled neutrophil cell membrane (magenta) and nuclear envelope (green). 30µm Z stacks were captured every 4.3 sec (0.26µm Z slice interval). Final video is a maximum intensity projection, LLSM data deskewed and deconvolved.