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1ST PRIZE: Melanie White, IMB at University of Queensland
Vasculature in a developing transgenic quail embryo. A transgenic quail embryo expressing LifeAct-EGFP was imaged live with an intact yolk and yolk sac in a dish.
2ND PRIZE: David Collings, Research School of Biology, Australian National University
Dendrobium speciosum. Roots from Dendrobium speciosum, the rock orchid found along much of Australia’s eastern seaboard, were fixed in formaldehyde / PBS and longitudinal sections cut by hand. After clearing, secondary cell walls containing the cross-linker lignin were stained with basic fuchsin. The living cells of the root cortex (left) contained a broad network of secondary wall thickenings whereas outside the exodermis (central band), dead cells of the velamen (right) showed a fine network of wall thickenings. The functions of these different secondary walls, and the way in which the different secondary wall patterns are generated by the cells, remain poorly studied.
DISTINCTION: Claire Richards, University of Technology Sydney
One organoid donut please. Placental organoids were generated by bioprinting trophoblast cells into a synthetic hydrogel using a RASTRUM bioprinter and maintained for 12 days. Organoids were harvested, fixed in 4% paraformaldehyde, dehydrated by tissue processing and paraffin-embedded for microtome sectioning. Sections were made at 5um thickness, transferred to slides and immunolabelled for cell adhesion protein E-cadherin (cyan hot) and counterstained with DAPI to visualise nuclei (orange hot).
DISTINCTION: Rosie Coleman, Flinders Health and Medical Research Institute
Sweet as Artificial Sugar. This image depicts a 3D cultured, murine small intestinal organoid exposed to the artificial sweetener, Equal. Organoids were fixed and immuno-labelled for Ki67, a cellular proliferation marker (Pink) and Epcam, a marker of epithelial cells (Multi-colour). Organoids were imaged in free suspension on a Zeiss LSM 880 Fast Airyscan Confocal Microscope. Image z-stacks were processed into a flat image using Image -J. The Epcam z-stacks were colour coded in Image J with the Zstack Depth Color Code plugin (using the “Fire” LUT) to help illustrate the 3D nature of the organoid in 2D. Colour was optimised with Adobe Photoshop.