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1ST PRIZE: Arnolda Jakovija, Garvan Institute of Medical Research
Ice and Fire. 2-photon imaging was performed in the ear pinnae of a transgenic mouse. This image shows the accumulation of myeloid immune cells (green) within the connective tissue (blue) and other components (red) of mouse ear skin. Multiple z-stacks were captured to cover the whole ear skin and stitched together. This transgenic mouse model allows us to study how immune cells move to sites of tissue damage in real time.
2ND PRIZE: Max Nobis, Garvan Institute of Medical Research
Metastatic Activity, Live and In Colour. In vivo imaging was performed with three channel FLIM of a Rac1-FRET expressing primary MMTV-PyMT breast cancer tumour (rainbow LUT) with fibrillar collagen I (Second Harmonic Generation, blue) and vasculature (Quantum Dots, red) visualised through a mammary imaging window. Activity of the metastatic protein Rac1 was mapped by the Rac-FRET biosensor. FLIM was recorded in three channels simultaneously on a Leica Stellaris system with an InSight DeepSee laser (Spectra-Physics) tuned to an excitation wavelength of 840 nm. Tile scan of the images was performed and stitched in Navigator (Leica) and a total area of 6285 x 6314 mm imaged.
DISTINCTION: Ryan Brown, Peter MacCallum Cancer Centre
Fishing For Blood: The Zebrafish Vascular Network. The zebrafish vascular network at 14 days post fertilisation. Two transgenic constructs allow the visualisation of the blood vascular network, Tg(kdrl:EGFP), and the lymphatic network, Tg(-5.2lyve1b:dsred2). Arteries are shown in red and lymphatics in grey, with veins indicated where the colours overlap. Zebrafish were anesthetised and mounted within low-melting agarose before live-imaging. Images were captured with the 20x objective on the Zeiss Elyra 780 Confocal microscope using the position function. Image processing was done on ImageJ and individual tiles merged on Adobe Illustrator.
DISTINCTION: David Hermann, Garvan Institute of Medical Research
Neutrophil Swarming. Multiphoton imaging of neutrophil swarming in the ear of a live RhoA biosensor mouse. Conditional biosensor expression in neutrophils was driven by LysozymeM-Cre (Lys-Cre). Neutrophil enrichment in the ear was achieved by injection of inactivated Staphylococcus aureus bioparticles prior to induction of multiphoton laser-mediated local tissue damage to stimulate neutrophil swarming. Biosensor fluorescence in neutrophils is shown in green with collagen-derived Second Harmonic Generation (SHG) signal in magenta.